The Cryo-EM Swedish National Facility offers access to state-of-the-art equipment and expertise in single particle cryo-EM and cryo-tomography (cryo-ET). The Facility has two nodes: at SciLifeLab in Stockholm and at Umeå University. Our aim is to provide:
SciLifeLab in Stockholm offers single-particle cryo-EM and cryo-ET of plunge-frozen samples, with a Talos Arctica for sample optimisation and a Titan Krios for high-resolution data collection.
The Umeå node offers single-particle cryo-EM and cryo-ET of plunge-frozen samples with a Titan Krios, Focused Ion Beam (FIB) lamellae preparation for cryo-ET with a Scios DualBeam SEM and sample optimisation support with a Talos L120. The Umeå node also offers a number of other TEM and SEM applications and sample preparation support, e.g. high pressure freezing, that can be accessed from http://nmisweden.se/.
The Facility is open to two types of applications: Rapid Access (RA) and BAG (Block Allocation Group). RA applications can be submitted at anytime, they are evaluated for technical feasibility and allocated time in the next quarter. BAG applications are evaluated once a year by a national Project Evaluation Committee, based on their scientific merit and technical feasibility. Microscope time is allocated 95% to BAG and 5% to RA projects. RA applications coming from institutions that have an already running BAG will be asked to join the BAG. The deadline for BAG applications is August 1st and they run from October 1st, every year. In order to submit an application, you need to register an account and log in. We invite you to read the information included in the 'Documents' tab.
Application Form required to apply for time on a project for either screening or data-collection. Machine time will be allocated on 24 or 48h slots.
Information required in order to submit request for group allocation time for one year.
2020/2021 BAG application call open
BAG (Block Allocation Group) call for CryoEM, cryoET and microED. We now open to BAG applications that allow users from one university to apply as a group and get a combined allocation that will extend for 12 months. You can find details about how a BAG works in the Documents session in the portal. You can apply for a BAG using the order form "BAG application - 2020/2021". The deadline is August 1st.
The Cryo-EM National Facility was established as part of the Structural Biology Platform at SciLifeLab by Gunnar von Heijne (Stockholm University) and Bernt-Eric Uhlin (Umeå University) and launched on September 1st 2016. The Facility is supported by the Knut and Alice Wallenberg, Family Erling Persson, and Kempe Foundations, SciLifeLab, Stockholm University and Umeå University.